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Here we clarify this ambiguous case by using sequence analysis and molecular modeling.

In addition, larval Anisakis specimens that were collected from anchovies (Engraulis encrasicolus) from the Tyrrhenian Sea, used for antigen preparation, were identified by using sequence analysis of the same genes.

We similarly analyzed water samples after concentrating pathogens by centrifugation at 3,000 × g for 15 min. We subtyped C. hominis and C. parvum by using sequence analysis of the 60-kDa glycoprotein gene (5 ).

To determine prevalence of H7, N9, H7N9, and H9N2 subtypes, we reviewed 48 peer-reviewed avian influenza surveillance studies in which sample sizes were stated and subtypes were nonselectively detected by using sequence analysis, reverse transcription PCR, or hemagglutination inhibition and neuraminidase inhibition assays.

In order to set up the central metabolism of P. falciparum with a focus on redox metabolism for building a network model, we used the database KEGG (http://www.genome.ad.jp/kegg) 34 and complemented the enzyme data produced by using sequence analysis, expert knowledge, and literature.

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Sequence data was collected by data collection software (Applied Biosystems), extracted using sequence analysis software (Applied Biosystems) and transferred to a UNIX workstation.

Subcloning efficiency DH5alpha bacteria (Life Technologies, Grand Island, NY, USA) were transformed with the ligated vector and colonies screened for correct insertion of hCXCR7 using differential enzyme digestions, followed by verification using sequencing analysis.

We also matched our set of prioritized targets witha study done by Ramakrishnan et al. [21] in which they predict DTIs by using sequence and structural analysis for understanding the evolutionary relationships between Mtb proteins and FDA approved drugs.

P. sojae and P. ramorum Δ-17 desaturases were identified by BLAST analysis using sequence of P. aphanidermatum Δ-17 as a query, against the P. sojae and P. ramorum databases of Department of Energy's Joint Genome Program (http://genome.jgi-psf.org/Physo3/Physo3.home.html).html

Approximate chromosomal locations of these three sex-determining regions have been inferred by Y-chromosome deletion mutant analysis using sequence tagged site (STS) markers (Bergero et al. 2008).

Substantial mutational analyses of BRCA1 and BRCA2 have been undertaken by site laboratories and, more recently, by Myriad Genetics using full sequence analysis [ 21], funded by multiple sources.

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