Sentence examples for by using sequence capture from inspiring English sources

One drawback of assaying variation by using sequence capture and mapping of short reads to the reference genome is that we are unable to comprehensively assay copy number variation caused by insertion of sequence not present in the reference.

This limitation points to future modifications of the approach by using sequence capture in order to enrich the cDNA for target sequences and to increase read depth for genomic DNA and RNA-seq (see below).

Using sequence capture followed by sequencing of a lane of Illumina Hi-Seq per library, we sequenced this interval in 5 pools of dogs each containing 5 samples from the same breed, resulting in average coverage of 4,227x.

Our preliminary data show that the enrichment of cDNA with sequences of interest using sequence capture methods (27) followed by high-throughput sequencing is an effective way to increase the read depth at transcripts of interest.

tridentata and vaseyana using sequence capture.

MI-1 SVM can be extended to solve the generic problem of binding site prediction by using sequence-based features that capture the non-contiguous nature of binding sites.

By using a sequence capture array that only targets Dscam, a more comprehensive cataloging of the expressed isoforms can be undertaken.

The soil functional diversity can be efficiently investigated by using the sequence capture technique that has been developed with an online web tool for probe design for genes encoding enzymes regulating specific functional mechanisms in ecosystems.

In these large genomes of 100 Mb to 10 Gb, computational speed and memory usage can become an issue, so reads that map to repetitive genomic regions are typically disregarded [ 6, 7, 9] or may even be removed from a sample prior to sequencing (e.g., by using exome capture).

vaseyana used for sequence capture.

As the genome of the Mediterranean mussel Mytilus galloprovincialis is not available at present, we have explored the possibility of reducing the whole genome sequencing efforts by using capture probes coupled with PCR amplification and high-throughput 454-sequencing to enrich selected genomic regions.