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Multilocus sequence typing (MLST) tools were used to delineate clusters of strains by using sequence output available from the MLST Web site (http://mlst.zoo.ox.ac.uk).ac.uk
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Each unique sequence for a locus was designated as a unique allele using Sequence Output (www.mlst.net).net
The typical user would most likely start out by using the sequence input/output API and the sequence/feature object model.
Regardless of the specific technologies used, sequencing assays are very complicated processes and the final output can be influenced by many factors including fluidics, optics, assay setup and sequencing library preparation.
The Samtools "view" method was used to further process sequence output.
In this paper, the problem of detectability is formulated as whether one can determine the current and all subsequent states of the NFTSs considered by using any sufficiently long input sequence and the corresponding output sequence.
In the prototype system we built, a high-speed oscilloscope was used to record the pulse sequence output by the SPC.
As with the screen in the complete sequence database TrEMBL we initially did not limit the BLAST output by using a restricting threshold.
This could be used with unparalleled efficiency to enhance sequence output of problematic amplicon libraries.
Then, the improved peak sequence output can be used for further analysis [ 81].
Using BSML as a common output format has the added advantage of regularizing data by using terms from controlled vocabularies such as the Sequence Ontology (Eilbeck et al., 2005) and Gene Ontology (Ashburner et al., 2000).
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