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The effects of these mutations were examined by monitoring expression of a firefly luciferase (luc) reporter gene under the control of NahR.

Macrophage activation was first evaluated by monitoring expression of genes coding for iNOS and Arginase I using RT-PCR.

One study, by monitoring expression of select immune genes, has indicated that N. ceranae but not N. apis may immunosuppress workers [ 32] at 7 days post-infection.

We confirmed that the night-time light period did not re-entrain the circadian clock, by monitoring expression of the clock gene LHY (see 'Materials and methods' and Figure 3 figure supplement 1).

To explore the potential relevance of our findings in the gp130 757F mouse model to human IPF, we first confirmed that excessive Stat3 activation in fibrotic lungs of bleomycin challenged gp130 757F mice was dependent on IL-6 by monitoring expression of the bona fide Stat3 target gene Socs3 (Kidder et al, 2008; Snyder et al, 2008).

To generate a PCF derivative of EATRO795 in which RNAi could be performed, the individual pLew29 and pLew13 plasmids were introduced by transformation, and controllable expression was tested by monitoring expression of a reporter gene subsequently introduced under the control of the T7 RNA pol promoter (data not shown).

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By monitoring expression levels of these gene sets, the impact of different diets on muscle composition and growth rate can be estimated from a single sample.

We monitored expression of mkp3 by means of mkp3-EGFP reporter fish, in which an EGFP cassette is inserted near the mkp3 gene by the sleeping beauty transposon.

The implication of ellagic acid on apoptotic process is further checked by monitoring expression and activity of caspase-3.

We validate the advantage of using IRES-containing bicistronic vectors, rather than monocistronic vectors, for long term expression of transgenes and show that one can deduce the expression of the first cistron by monitoring the expression of an IRES-driven second reporter cistron in vivo.

These results correlated with the Stat1-driven gene expression as assessed by monitoring the expression of Stat1-mediated IFN-inducible 6-16 mRNA.

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