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We demonstrated the more general applicability of this method to dynamically track receptor-mediated phagocytosis by monitoring internalization of FITC-labeled fibronectin beads in NIH 3T3 fibroblast cells.
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What is the nature of the interactions that are disrupted by internalization?
We followed integrin α5β1 endocytosis by monitoring the internalization of the anti-α5β1 antibody P8D4 (Figure 8).
To monitor internalization more directly, cell surface HA-GnRHR were Ab loaded by incubation for 60 min at 21°C, before washing and incubation for varied times at 37°C in the presence or absence of agonist.
This pathway was further investigated by monitoring the cellular internalization of fluorescent LDL-[BODIPY-Cho].
We next sought to determine whether celastrol inhibited either toxin internalization or the enzymatic activity of LF by monitoring cleavage of MAPKK1, a cytosolic target of LF.
We monitored the internalization of the fluorescent complexes 47· 48 and CB[7]· 48 into murine lymphocytic leukemia cells (L1210) and their derived cell line L1210FR, which overexpresses biotin receptors, by confocal microscopy and flow cytometry.
Using a GLP-1 conjugated to tetramethyl rhodamine to monitor the internalization of the receptor, Kuna et al. [ 37] observed that after internalization, GLP-1R/ligand complex could be co-localized with adenylate cyclase in the endosome.
Cellular internalization of the polyplex formulations was determined by monitoring Cy5-labeled pDNA.
The internalization efficacy and bioavailability of encapsulated DQ-ovalbumin were assessed by monitoring the fluorescence changes in the cargo.
Validation of GFP-tagged CXCR4 receptor activity was done by monitoring receptor internalization post CXCL12 stimulation (Supplementary Fig S6b).
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