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The ability of chaperones to help proteins refold in vivo can be assessed by monitoring reactivation of a thermolabile bacterial luciferase after exposing cells to elevated temperature.
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To identify factors that might be acting directly to maintain epigenetic silencing, we performed analyses for a set of 20 factors to measure the rapidity of the response by monitoring GFP reactivation profiles (Fig. S3).
We therefore established two epiSC reporter lines to examine reprogramming by monitoring the status of X reactivation and by analyzing the activation of Oct4 DE in response to germline factors (see also later).
Next, we established a homogeneous population of GFP-negative epiSCs, termed XiGFP epiSCs, in which the GFP transgene is located exclusively on the Xi, providing a basis to study the reactivation of the Xi by monitoring GFP expression.
Thus, by monitoring mRNA levels over a reactivation time course, direct and secondary NMD targets can be distinguished.
19 20 25 Moreover, prompt initiation of treatment, plus regular monitoring for reactivation of disease and/or second eye involvement, are critical to improving outcomes.
Reactivation of the transgene is easily scored by visually monitoring accumulation of anthocyanin pigmentation.
Herpes zoster is caused by the reactivation of varicella-zoster virus from sensory neurons.
The disease, caused by a reactivation of the virus that causes chickenpox, results in a painful rash and blisters, with potentially serious complications.
Shingles is caused by the reactivation of the chicken pox virus and results in a nasty skin rash and fever.
According to Okumura (2016), this series of earthquake shocks were triggered by the reactivation of the Futagawa and Hinagu Faults.
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