In parallel, media samples were taken to track expression of construct 4 by PAGE and western blot (Fig. 5A, B).
We used two independent approaches to track expression of individual exons and mRNA isoforms, and we connected changes in expression to functional consequences by mining a variety of databases.
Applicability of the assay was evaluated by tracking protein expression of two GST-fused allergens of biopharmaceutical value in E. coli, GST-Der p 2 and GST-Mus a 5, both targeted to inclusion bodies.
By tracking the expression of an embryonic Nkx2.5 cardiac enhancer, we identified cardiomyoblasts capable of differentiation into striated cardiomyocytes in vitro.
To fit the puzzle together, a team led by Catherine Thompson, a molecular biologist at the Kennedy Krieger Institute and Johns Hopkins University School of Medicine in Baltimore, Maryland, started by tracking the expression of Hairless in healthy follicles.
We examined HDAC2 activity in THI-treated mdx muscles by tracking the expression of its target genes, especially miR-1 and miR-29.
A gene called Arc is known to turn on when the neuron fires--so by tracking its expression in this region of the brain, the team could determine which neurons switched on in response to specific smells.
Having precisely established the key cell-cycle and cellular parameters accompanying differentiation during early infection, we monitored transmission competence by tracking the relative expression of PAD1 mRNA by qRT-PCR in each mouse on each day of infection.
MT4-MMP null mice were described previously [8] and because part of the mouse MT4-MMP genomic locus was substituted with the bacterial LacZ gene we were able to monitor of MT4-MMP expression by tracking β-gal expression.
Preliminary work on facial expressions, by tracking the motion of 20 identified spots, has been done by Suma et al.[13].[13]
