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It has been demonstrated that the cumulative effect of pesticides, even at doses below the detectable threshold, weakens bees and causes significant mortality in the colony [1].
Part of the discrepancy between mRNA abundance, protein content, and enzyme assays may be that multiple small changes in interacting and synergistic pathways/factors combine to influence flux through metabolic pathways at the protein level, and that each change is below the detectable threshold for statistical significance.
In glomerular cells, the levels of MCAD and GRP-75 proteins were below the detectable threshold.
Rare variants with larger effect sizes (OR, ≥2), but present at frequencies below the detectable threshold of GWAS (<5%), have been hypothesized to explain part of this "missing" genetic effect [ 8, 9].
Where possible, we used the reported data to compute a 95% confidence interval (CI) for mortality, immunological response (increase of CD4 cell count) and virological response (proportion of patients with VL below the detectable threshold).
Our microarray profiling data for the embryos (Additional file 13) also identified three of these genes (DSTN, PAIP1, UBE4B), while the spot intensity for NASP was below the detectable threshold.
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It also illustrates the utility of an analytical approach that incorporates exclusion of data for transcripts that do not generate detectable signals; so that those genes below the detectable thresholds are readily identified, rather than using 'flooring' or other methods for filling in low intensity or empty microarray data fields.
The two remaining cystatins, Glyma13g25870 and Glyma14g04250, did either not change (Glyma13g25870) or expression was below, or close to, the detectable threshold level (Glyma14g04250).
In conclusion, while the maximum detectable flow speed of PR-DOCT can be extended through the shortening of the acquisition time interval, the minimum detectable velocity will also be increased; this approach leads to the invisibility of the slow flow activity whose axial speed is below the minimum detectable threshold of the system.
It can be observed that certain speed of slow flow fell below the minimum detectable threshold of the VDR1 as demonstrated in Fig. 5(b).
Interestingly, whereas MMP9 was below the detection threshold of qPCR assay with AA-aRNA and the original RT protocol (Cq > 35 cyles), protocol optimization was able to make it detectable.
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below the visible threshold
below the apparent threshold
below the detectable limit
below the sensor threshold
below the detectable thresholds
below the detectable concentration
below the detectable range
below the acceptable threshold
below the detectable size
below the detectable level
below the detectable limits
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