Sentence examples for before being heated at from inspiring English sources

Then, 2.5 μl of catalyst ink was coated onto the GCE using a micropipette and dried for 1 h at room temperature before being heated at 80 °C for another 30 min.

Then, 20 mg of sodium dithiobenzoate (in 0.5 mL; pH = 7) was added before being heated at 100°C for 30 minutes, which allowed for the formation of the 188Re-SSS complex.

The mixture was degassed for 20 min, before being heated at 90 °C for three days.

The plates were then air-dried and sprayed with an alcoholic solution of α-naphthol before being heated at 150°C.

The transfected cells were washed with 1X PBS and mixed with an equal volume of a 2X sample buffer [0.5 M Tris (pH 6.8), 100% glycerol, 10% (w/v) SDS, 0.5% (w/v) bromophenol blue, 10% (v/v) β-mercaptoethanol] and short spun before being heated at 100°C for 10 minutes.

The hydrolysis process was conducted at the required period before being heated rapidly and maintained at a temperature of 90 °C for at least 10 min (Aleman et al. 2011) to deactivate the enzyme.

The vial was sealed with a rubber septum and purged with N2(g) for 2 h before being heated in a hot oil bath at 70 °C for 1 h.

Ten mL of extraction buffer (2 % CTAB, 2.5 % polyvinylpolypyrrolidone, 2 M NaCl, 100 mM Tris HCl pH 8.0, 25 mM EDTA pH 8.0 and 2 % of 2-mercaptoethanol added just before use) was heated at 65 °C in 50 mL tubes.

The extraction buffer containing 2% CTAB, 2% PVP, 100 mM Tris HCl pH 8, 25 mM EDTA, 2 M NaCl, 0.5 g/L spermidine and 2% β-mercaptoethanol (added just before use) was heated at 65°C for 10 min in a water bath.

Before measurements, the samples were heated at 180 °C for 24 h.

Before sublimation, the samples were heated at 1150°C for 10 min to remove any trace of native oxide.