Sentence examples for before being fixed at from inspiring English sources

(It lost a further 21% this year, before being fixed at 24,975 to the dollar).

Cells were allowed to settle on a poly-l-lysine coated coverslip for 20 min before being fixed at a final concentration of 2% PFA.

Staining of lipids with Oil Red O was performed using the propolene glycol (PG) method: 12-μm fresh frozen sections were cut and air-dried at room temperature (RT) before being fixed at 4°C in 10% formalin for 5 minutes.

In addition, we have used a set of central PSFs modelling the Wide Field Camera of the Hubble Telescope in the year 1991, before being fixed, as a realistic (and, at a certain moment, useful) SV restoration problem.

The cells were then incubated with CD1d/α-GalCer tetramer (ProImmune) for 20 min on ice followed by incubation with anti-B220 and anti-TCR-β antibodies for 15 min before being fixed overnight at 4°C in 4% formaldehyde (Sigma-Aldrich).

After incubation, cells were collected from wells with trypsin and pelleted by centrifugation at 6000 rpm for 5 min, before being fixed with 4% paraformaldehyde at 4°C.

Then the cells were washed again with PBS before being fixed with 70% ethanol at −20C overnight.

Then the cells were washed again with PBS before being fixed with 70% ethanol at −2°C overnight.

RAW264.7 cells and BMDM (0.25 × 10/well of a 12-well plate) were collected and washed in cold Dulbecco's Phosphate Buffered Saline (DPBS) before being fixed with 70% ethanol at -20°C.

Cells were centrifuged at 1200 rpm at 4°C and washed twice with cold 1× PBS before being fixed with cold 70% ethanol and kept at −20°C before staining.

After treatment, conceptuses were incubated in KSOM-AA medium for 30 minutes at 37°C before being fixed and processed as described in previous section.