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Serum obtained after centrifugation was immediately frozen and stored at −80 °C before being analyzed at the phenotyping platform (GenoToul Anexplo, Toulouse, France).
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He said the powder was being analyzed at a lab.
Adult flies were continued to be raised at 17°C for about 7 days before being analyzed for their cardiac function (at RT).
Samples were incubated for at least 30 min at 37 °C before being analyzed by flow cytometry.
Before being analyzed, soil samples were dried at room temperature and sieved (2 mm).
The autosampler storage stability was determined by storing the reconstituted QC samples for ∼ 48 h under autosampler condition (maintained at 8°C) before being analyzed.
Post-preparative stability was determined by storing the reconstituted QC samples for ∼ 48 h under autosampler conditions (maintained at 10°C) before being analyzed.
The autosampler stability was determined by stored reconstituted QC samples for ~72 h under autosampler condition (at 10 °C) before being analyzed.
An aliquot of each calibration standard was then digested 1 1 with nitric acid and incubated overnight at 37 °C before being analyzed for its metal concentration by ICP-MS.
Samples were mounted on stubs and sputter coated with gold before being analyzed by scanning electron microscopy (SEM) at 15 kV (SSX-550; Shimadzu, Tokyo, Japan).
Samples were stored at −70 °C before being analyzed.
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