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Then, 0.1 mL (broken-) filtered broth was added to the above solution before being assayed at 500 nm using a visible 721 spectrophotometer.
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The sample was stored at -80°C before being assayed.
Samples were collected in EDTA, separated, and stored at −20°C before being assayed.
Before and after samples were assayed at the same time for folate, insulin, FFA, triacylglycerol and faecal calprotectin.
VV was assayed before and at 5 day intervals after initiating dextran infusion, immediately prior to manipulations of the infusion status (On/Off).
Mice received an injection of glucose (2 g/kg body wt), and blood glucose was assayed immediately before and at the indicated time after glucose administration.
Mice received an injection of human regular insulin (2 units/kg body wt; Novo Nordisk, Copenhagen, Denmark) into the intraperitoneal space, and blood glucose was assayed immediately before and at the indicated time after injection.
In both groups the serum concentrations of ferritin and hs-CRP were assayed before surgery and then at two and six weeks after surgery.
The animals were injected with human regular insulin (0.75 U/kg of body weight) into the intraperitoneal space, and blood glucose was assayed immediately before and at 20, 40, 60, and 80 minutes after injection to determine the insulin sensitivity.
Cells were plated on coverslips at least 2 days before the assay and incubated in serum-free culture medium at least 24 h before nuclear import of GFP-ERK2 was assayed.
And I guess what I'm assaying is we here at the White House agree".
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