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With these models we can perform a variety of experiments that could previously only be done in cell culture or in prostate cancer cell line xenografts.

Furthermore in vitro and in vivo specific studies will be done in cell line and tumor bearing mice to confirm the usefulness of this chitosan backbone for FR targeting agent design.

The mechanism by which cholesterol regulates Kir channels has remained elusive since, until recently, experiments could only be done in cell lines where membrane lipid compositions are undefined, difficult to manipulate quantitatively and can vary dramatically from cell to cell and with time.

However, well before this stage, preliminary studies confirming the potential probiotics' mechanisms of action need to be done in cell and animal models.

Where FLIM FRET proves most advantageous, is that it is generally insensitive to the relative concentrations of the fluorophores tagged to the proteins of interest and can be done in cell lines transiently expressing either one or both of the fluorophore-tagged proteins.

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In this Primer, we explain the basic principles of AFM and provide a snapshot of some of the exciting work being done in cell biology using this multifunctional tool.

Incubation was done in cell culture conditions for 1 month.

Furthermore, most of this work has been done in cell lines such as COS1, HeLa, and NIH3T3 cells [33], [40], [51].

Beyond this, the majority of the experimental evolution studies have been done in cell culture systems which are generally not accurate models of natural host systems.

Incubation was done in cell culture conditions for 1 month, during which time mineral precipitates appeared gradually that were then sedimented, washed, and processed for gel electrophoresis and Coomassie blue staining.

Further dilution was done in cell culture medium as required.

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