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This resistance can be mimicked in cell culture by ectopic overexpression of bcl-2.
The initial stage of AChR clustering at developing NMJs in intact embryos involves the formation of ovoid aggregates (3), and can be mimicked in cell culture by applying a soluble recombinant form of neural agrin to myotubes (8, 9).
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Experimentally, such conditions can be mimicked in other cell types by exposure to hypoxic, acidic, ion-shifted Ringer followed by return to normal solution.
If that natural mutation could be mimicked in human blood cells, patients could be endowed with immunity to the deadly virus.
67b A recent study has shown that the formation of RNA granules lacking a boundary membrane can be mimicked in vitro by incubating cell or tissue lysates with a biotinylated isoxazole reagent (b-isox).
ECs are the primary cells involved in the formation of new blood vessels or angiogenesis and this can be mimicked in vitro by culturing cells on matrigel [ 29].
We report here that some molecular and functional adaptations of CR, such enhanced stress resistance, can be mimicked in vitro by culturing cells with serum from individuals practicing long-term CR without malnutrition.
This enhanced long-term survival could be mimicked in wild-type MEF cells subjected to ER stress by the addition of Boc-D-FMK (Fig. S1C) indicating a blockade in caspase activation can exert a long-term survival benefit following ER stress.
Whereas these two core changes could also be mimicked in vitro, DNA relaxation/decondensation occurred upon cell surface binding only, suggesting that this is a specific process induced by receptor binding.
This cell proliferation can also be mimicked in vitro.
EpH4 cells are nontumorigenic cells derived from spontaneously immortalized mouse mammary epithelial cells [ 14], which can be used as a mammary gland model system, since the initial stages of mammary gland differentiation can be mimicked in vitro [ 15] by treating EpH4 cells with the steroid hormone dexamethasone (Dex).
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