At 10.53pm, another woman was placed in cell four.
Once counted, the cells were placed in cell culture flasks at a concentration of 107 cells/ml.
Renal cortices were placed in cell lysis buffer and protease inhibitors on ice.
Immediately after polymerization, gels were placed in cell culture medium to rinse out unreacted monomer and photoinitiator (30 minutes).
The cell suspension was placed in cell culture flask (∼10 cells per flask), and growed at 37 °C in humidified air with 5% CO2 until confluent.
Following fabrication, hydrogels were placed in cell culture medium at surface area:fluid volume ratio of 3 cm/mL and incubated for 24 h at 37 °C.
Prior to cell seeding, a drop of the barrier gel was placed in cell culture wells in order to test inhibition of adherence and proliferation or coated over a polymerized collagen gel to assay for prevention of invasion.
The epithelial layer and lamina propria were separated from underlying stroma and the tissue explants (8 mm) were placed in cell culturing plates containing RPMI 1640, 20 μg/mL Gentamicin, 10 mM HEPES (Fisher Scientific, Gothenburg, Sweden), and 5% pooled HS Fisher Scientificc).
The infected breast cancer tissue slices and human liver tissue slices were placed in cell culture lysis buffer (Promega) and homogenized with an ultra sonicator (Fisher Scientific Model 100, Pittsburgh, PA, USA) at a setting of 15 watts for 10 s.
Unlike the older inmates in Onomichi Prison, those in Shizuoka are placed in cells with inmates of various ages.
