Sentence examples for appropriate as previously from inspiring English sources

It also involves immunohistochemistry for tau, α-synuclein and ubiquitin, as appropriate, as previously described [ 4].

Screening newly referred patients for diabetes and where appropriate referring them to a diabetes specialist would appear appropriate as previously suggested [ 38].

The threshold aims to reduce the inclusion of false positive responses and the frequency distribution data from the prevaccination responses among these teenagers indicates that the response threshold is appropriate as previously described [ 10, 13].

Immune pellets were precipitated by the addition of protein A Sepharose and analysed by immunoblotting using anti-FLAG, anti-c-Myc and anti-His antibodies as appropriate as previously described (e.g. [14]).

The CD18 gene was sequenced using the appropriate primers as previously described [21].

For double or triple staining, 1×106 cells were incubated with the appropriate MoAbs as previously described [29].

Embryos from each of the wild-type D. melanogaster, and mutant Abd-BT2N, Abd-BLDN and Abd-BΔPTE-UP lines were collected, fixed and hybridized with the appropriate probes as previously described [32].

After completion of the recording, each neuron was incubated with 10 µg/ml IB4-FITC (Sigma-Aldrich, St . Louis MO) for 10 minutes and visualized with appropriate filters as previously described [21].

Aliquots of equal volume were examined using western blot analysis for the presence of sema3s using antibodies directed against the appropriate tags as previously described [19] and the densitometry analysis was preformed using MultiGauge software (FUJIFILM).

Cytokines were measured at the Luminex Core Facility (Utrecht, The Netherlands) with a multiplex immunoassay (Luminex, Austin, TX, USA) using an in-house-validated panel which incorporates an appropriate Quality Control Program, as previously described [23].

cDNA was prepared by reverse transcription with appropriate controls as previously described [ 29].