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Unfortunately this analysis, as done before [4], requires definitive outcome data on the whole cohort of patient, and thus a minimum of a 5-year follow-up study will be necessary as previously done [4].
It further confirmed that there was no sample processing bias due to irradiation state and that raw data normalization was not necessary as previously proposed [ 26].
All positions showing differences to the best-scoring reference sequence were visually inspected in the electropherogram, and the sequence was corrected manually if necessary as previously described [ 45].
In addition, PCT daily measurement is used, in addition to clinical judgment, to assess prognosis, to predict unfavorable outcome and to customize the length of antibiotic therapy if necessary, as previously published [ 7].
In patients who received NNRTI or PI drugs as part of their therapy, plasma levels were monitored and adjusted to therapeutic drug levels when necessary as previously described [ 14, 15].
Positional C enrichment for metabolites was determined by integration of appropriate central and C satellite peaks in one-dimensional and two-dimensional TOCSY experiments, followed by corrections for differential relaxation where necessary as previously described [ 8, 11].
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Oligonucleotides were purchased from IDT, purified by denaturing acrylamide gel electrophoresis and end-labelled with [P]ATP where necessary, as described previously [ 19].
Glucose tablets were provided when necessary and as previously described (20).
The GP will provide usual care conform the NHG guideline on depressive symptoms when deemed necessary [ 46], as previously described in the intervention strategy paragraph.
This may seem a priori impossible since these columns or equivalently the corresponding vectors (left {boldsymbol {r}^{(l)}_{boldsymbol {theta }}right }_{l=N_{a}-2K}^{N_{a}})—do not indeed contain the necessary 2K+1 cross-covariances as previously required.
Around the time of the Dissolution of the Monasteries this duty became even more necessary as the monasteries were previously repositories of local genealogical records.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com