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Exact(19)
Statistical analysis and graphs were performed with GraphPad Prism software (GraphPad Software Inc .. Non-parametric Mann-Whitney tests were used to compare tumor lymphatic vessel coverage of treated versus control mice.
Tumor macrophage depletion and tumor lymphatic vessel coverage were determined by immunofluorescence stainings with anti-F4/80 antibodies and anti-LYVE-1 antibodies, respectively, and ImageJ software (http://rsb.info.nih.gov/ij/).
However, vessel coverage in the retina was unperturbed in the Nrp1 VEGF− mutants, n = 6.
It is well known that tumor vessels are less-covered compare to normal vessels, and increasing evidence suggests that vessel coverage affects tumor vessel functionality [ 29].
In line with these reports, Wnt1 enhances tumor vessel coverage in gliomas via increasing endothelial PDGF-B secretion, whereas DKK1 inhibits this effect [ 32].
One limitation of the vessel coverage analysis is that it is two-dimensional, and axial re-modeling is not completely captured.
Similar(41)
In mouse xenograft models of human ovarian and esophageal cancers, tumor vasculature normalization with bevacizumab treatment results in the increase of vessel pericyte coverage [ 20].
Due to the large number of αSMA staining myofibroblasts the difference in vessel pericyte coverage is only an estimate and may be underestimated since not all vessels in the periphery stain for CD34.
Because the new stent has high vessel-surface coverage, the arrangement of the wires may work as an extraordinary tight mesh that stabilizes the plaque, thereby preventing embolic complications.
PDGF is expressed by endothelial cells and acts in a paracrine manner by recruiting PDGFR-expressing cells, such as pericytes and smooth muscle cells, to the developing vessels, thus improving pericyte coverage and vessel function.
The Nrp1 VEGF− mutant mice display normal vessel branching and coverage at postnatal stages.
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