Sentence examples for vector cdna from inspiring English sources
To construct a Myc-tagged Beclin1 expression vector, cDNA encoding the full-length 1353-bpp long) human Beclin1 (GenBank ID: NM_003766.3) was amplified by reverse transcription-PCR (RT-PCR) from human mesenchymal stem cells (MSCs) and cloned into the pCMV-Myc vector (Clontech).
Plasmids were from the following sources: EYFP, Matchmaker© and Myc-CMV vector cDNA from CLONETECH; and human M5 muscarinic receptor cDNA from L. Birnbaumer (NIH).
NS assisted with cell tracking, while SB cloned PMMs into entry vectors, and SW supervised SB and generated entry vector cDNA libraries.
The In-Fusion reaction was performed by combining 5X In-Fusion reaction buffer, pSMART2IF linearized vector, cDNA, In-Fusion enzyme, and ddH20 and then incubating at 15 min 37 C followed by 15 min 50 C.
To construct the pBS-NFCas9a Cas9 expression vector, cDNA corresponding to the open reading frame (ORF) of hCas9 was first PCR-amplified using phCas9 (#41815; Addgene, Cambridge, MA, USA) as template.
For the construction of the β-catenin/TCF reporter plasmid with GFP (TOPEGFP vector), cDNA encoding EGFP was excised from pIRES2-EGFP (Clontech) and replaced with luciferase gene in TOPFLASH plasmid (Upstate).
To construct expression vectors, cDNA fragments were amplified by PCR.
DCs can be pulsed with tumor-associated antigen through viral vectors, non-viral vectors, cDNA, or mRNA.
To generate pAG3-6 × stops-ATG- GA 50-3T vectorstops-ATG- GA 50-3T50 stops-ATG- GA 50-3Tthout an ATG start codon was inserted in a pAG3-6 × stops-3T vectorsbetween the 6 stop cDNAns and the 3 tags (ofline Resource 4a).
Such a phenomenon can explain in part but other possibilities may also exist for the loss, such as the limitation of cloning efficiency when ligating cDNAs into vector during cDNA library construction, and clonal loss during library transformation etc.
O432 cells were transfected with prostasin siRNA duplexes (Ambion, Austin, TX, USA) and O432-RP cells with prostasin or PAK2-p34 cDNA-containing vectors (cDNAs were amplified by reverse transcription PCR and then cloned into a pCI-neo vector) using Lipofectamine 2000 (Invitrogen) according the manufacturer's instructions.
