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Exact(20)
Variant bands were subsequently cut from gels, resuspended in distilled water, subjected again to PCR, and then sequenced.
Meanwhile, 16.72% were variant bands (7.47% were hypermethylation, type C; 9.25% were hypomethylation, type D).
The cloned variant bands were sequenced with vector primers by automated sequencing.
The detection of characteristic variant bands indicated the presence of the mutation being studied.
Variant bands were reamplified and used for direct sequencing on ABI Prism 3730 DNA analyzer (Applied Biosystems).
Where variation was found, variant bands were reamplified from the gel and sequenced by Big Dye sequencing on an ABI prism 377 sequencer.
Similar(40)
Also as expected, the 3HA-tag Hsp90 variants form bands of slightly higher molecular mass that are distinct from the endogenously encoded Hsp90 (Fig. 4D).
Noise and motion are nonuniformly distributed throughout an image; correspondingly, we used the signal and noise characteristics of each individual voxel to generate a scan-specific and spatially variant band-pass filter.
The variant band is either smaller or larger.
The mixed population CGD2.GC16A showed both the exon-2-skipped variant band as well as a faint WT band from the corrected mRNA.
The amount of each V. proteolyticus variant in a sample can be obtained relative to E. coli, e.g. as a ratio, by comparing the V. proteolyticus variant band density with those obtained on the standard curve.
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