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Their mechanism of action involves the ATP-dependent transport of one DNA duplex through a transient break in a second DNA duplex.
Topos carry out an ATP-dependent strand passage reaction whereby one double helix is passed through a transient break in another.
A second DNA duplex termed a 'transported' or 'T' segment can be captured by the N-terminal domain of the protein, and passed through this transient break in a 'gate' or 'G' segment.
Their mechanism of action involves the ATP-dependent transport of one DNA duplex through a transient break in a second DNA duplex; metal ions are essential for strand passage.
Supercoiled DNA undergoes a topological rearrangement when the catalytic Tyr723 binds the scissile strand DNA 3' terminus thus introducing a transient break in the phosphodiester chain (catalytic mechanism B).
The T-segment is then passed through a transient break in the G-segment (opened by the N-terminal ParC (GyrA) domains), the DNA is resealed and the T-segment released through a protein gate prior to resetting of the enzyme to the open clamp form.
Similar(51)
Therefore, there was a minor transient break-in during the TER measurement after nanoparticle addition and a recovery of the TER could be observed (Figure 4).
These enzymes control DNA topology by introducing transient breaks into DNA strands.
Type II topoisomerases make transient breaks in double-stranded DNA (dsDNA).
Topoisomerases are ubiquitous proteins which have the function of manipulation of the topological structures of DNA by generating transient breaks in the double helix in the cells [ 1– 4].
DNA topoisomerases control DNA topological states by catalyzing the transient breaking and rejoining of single strand DNA, allowing DNA strands or double helices to pass through each other [ 27].
Related(20)
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