Sentence examples for transformation kit from inspiring English sources

The recombinant plasmids and the empty plasmid were used to transform Z. bailii IST302 using a commercial yeast transformation kit (MP Biomedicals, California) with minor modifications.

This plasmid was digested with BamHI and BglII, and the linear DNA fragment with BamHI and BglII ends (Fig. S1A in Supporting Information) was transformed into yeast using a Frozen-EZ Yeast Transformation kit II (ZYMO Research).

Plasmid DNA to be assayed was transformed into yeast strain MRG5 [ciro] using Frozen-EZ Yeast Transformation Kit II™ (ZYMO RESEARCH), plated on SC, -Leu, 2% glucose plates and incubated at 30 °C.

EasyComp™ transformation kit according to the manufacturer's instructions (Invitrogen), and separately transformed with each of the five C. gibbosum CYP expression clones and the control Arabidopsis β-glucuronidase (gus) gene.

Plasmids were transformed to competent bacterial cells using the Mix & Go E. coli Transformation Kit (Zymo Research) or supercompetent XL1-Blue cells according to the manufacturer's instructions.

The resulting 1.2-kb PCR product was cloned into the vector pYES2 and transformed into the Saccharomyces cerevisiae strain DBY747 using the Frozen-EZ Yeast Transformation kit (Zymo Research, CA).

Saccharomyces cerevisiae W303-1A was transformed with the constructed plasmids using the Frozen-EZ Yeast Transformation II kit (Zymo Research, Irvine, CA, USA).

BD and AD Gateway compatible vectors pNLex and pB42AD were co-transformed into yeast EGY48/p8op-lacZ (Clontech) by using the Frozen-EZ yeast transformation II kit (Zymo Research).

Yeasts were transformed with a plasmid encoding HA-Snf1 using the Frozen-EZ Yeast Transformation II Kit (Zymo Research, T2001) according to the manufacturer's instructions.

Saccharomyces cerevisiae FY834 (MATα; his∆200; ura3-52; leu2∆1; lys2∆202) was refreshed on YPD agar medium (per liter: 20 g glucose, 20 g peptone, and 10 g yeast extract) at 28 °C for 48 h and then used for preparing competent cells as described by Frozen-EZ Yeast Transformation II Kit (T2001, Zymo research, USA).

EasyComp transformation™ kit (Invitrogen, CA, USA).