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Cell cycle distribution was determined by the Click‐It EdU incorporation Kit (Life Technologies).
Cell-cycle distribution was analyzed by Click-It EdU incorporation Kit (Invitrogen) according to manufacturer's guidelines.
The Genisphere 900 3DNArrayay Detection (Genisphere Inc., Hatfield, PA) indirect incorporation kit was used to generate cDNA samples for hybridization.
The cell growth was measured using BrdU incorporation kit from CellSignal (Danvers, MA, USA) and followed the manufacture's procedure or MTT assay.
Proliferation rate was analyzed after 3 days of co-culture using 5-bromo-2'-deoxy-uridine (BrdU) incorporation kit (Roche, Basel, Switzerland).
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hUCB-MSC proliferation was evaluated using a BrdU incorporation assay kit (Amersham; Cell Proliferation Biotrak ELISA System; RPN250; GE Healthcare, Little Chalfont, UK) according to the manufacturer instructions.
Immunohistochemistry staining of bromodeoxyuridine (BrdU) was performed by using a BrdU incorporation assay kit (BD Biosciences).
Cell proliferation assay was performed with BrdU incorporation assay kit according to manufacturer's protocol (Roche Diagnostics GmbH).
To analyze the effects of the SMI vemurafenib, perifosine, regorafenib, and trametinib (all from Selleckchem, Houston, TX, USA) on cell proliferation, DNA synthesis was measured using a 5-bromo-2′-deoxy-uridine (BrdU) incorporation assay kit (Roche Applied Science, Mannheim, Germany).
We found that SM had no apparent effect on total BrdU incorporation in Kit-positive spermatogonia cultured for 24 h.
The effect of LACE or CE on tumor cell proliferation was assessed by measuring BrdU incorporation with the kit described above.
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