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The harvested fat was immediately processed in the Lipogems® processing kit (Lipogems International Spa, Milan, Italy) as previously described (Bianchi et al. 2013).
Process the plate according to instructions that accompany the holography processing kit.
The 10 bioarrays, in this study, were processed in parallel using the CodeLink Shaker Kit and CodeLink Parallel Processing Kit (Amersham Biosciences).
Tissue homogenates were prepared according to the procedure described in Affymetrix QuantiGene Plex sample processing kit for FFPE tissues (Panomics Inc).
Consider purchasing a biofuel complete ready-to-run biofuel processing kit.
Make sure to read thoroughly and follow all instructions and warnings that come with the processing kit.
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In addition, 200-300 mg of cartilage from a non-weight-bearing portion of the knee, generally the medial or lateral superior ridge of the condyle or the intercondylar notch, was collected and sent to the GMP-certified, cell-culturing facility (SewonCellontech, Korea) for processing using the CE/MDD-certified, human-cell-processing kit (CRM kit™, SewonCellontech, Korea).
Quantification was performed employing a Image processing tool kit (Adobe Photoshop) software that utilizes a color subtractive process [76].
Adipocyte cell size was measured on digital microscopy images using Adobe Photoshop CS2 (Adobe Systems, San Jose, CA, USA) and Image Processing Tool Kit (Reindeer Graphics, Asheville, NC, USA) as described previously [4].
The histology sections was viewed at 10× magnification, and images were obtained with a SPOT digital camera (Diagnostic Instruments, Sterling Heights, MI) and converted into a binary format with Adobe PhotoShop 6.0.1 (Adobe Systems, San Jose, CA) and Image Processing Tool Kit (Reindeer Graphics, Gainesville, FL).
Researchers found there were no established procedures for processing the kits.
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