Exact(3)
We have recently identified a new family of multidomain oxidoreductase (redox) enzymes, the MICALs, that directly regulate the actin cytoskeletal elements necessary for the morphology, motility, and trajectory of cells.
To test the role of p38 in BMP-2-induced cell migration, we performed an in vitro migration assay using time-lapse imaging and analyzing the trajectory of cells for 16 hours (Movies S1 and Movies S2).
We also analyzed the ability of the phospho-mimetic mutant of Hsp27 to affect the p38/MK2 signaling by in vitro migration assay using time-lapse imaging and analyzing the trajectory of cells transfected with Hsp27-EEE for 16 hours.
Similar(5)
Examples of "winds" could include externally applied cytokines or drugs that transiently alter network components and thereby influence the trajectory of cell phenotype changes, for example, differentiation.
We discuss the consequences of ordered pseudopod extension for the trajectories of cells, and for the mechanisms that cells may use to respond to external cues such as chemoattractants.
By using the model, the trajectories of cells in the channel under acoustic standing wave excitation which showed different metastatic capacities were calculated.
The similar movement trajectories of cells can also be seen in ESI Video S2. † It is interesting to track the dynamics in the electrophoretic mobility of a cell under the pulse sequence and the local pH at the moment.
This approach will be particularly powerful in revealing how the developmental trajectories of cells and circuits are altered, what are the primary cell autonomous changes due to 'direct hit' of the mutant gene, and what are the secondary, possibly maladaptive changes that lead to aberrant circuit-level operations.
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Justyna Jupowicz-Kozak
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