Sentence examples for the appropriate minute from inspiring English sources

Following 500 million years of evolution, approximately three dozen cell-specific intracrine enzymes have been engineered in human peripheral tissues whereby the inactive sex steroid precursor DHEA mainly of adrenal origin is transformed into the appropriate minute intracellular amounts of androgens.

"We are not looking at it that way now because the players' fitness is pretty good and they have had the appropriate minutes on the clock".

One unit (U) of enzyme activity was defined as the amount of enzyme required to liberate 1  μmol of reducing sugar from the appropriate substrate per minute per mL of crude filtrate under the assay conditions.

Sedimentation equilibrium experiments were performed using Epon charcoal-filled six-sector centerpieces at the appropriate revolutions per minute with 0.001 cm spacing, scanned every 4 h, and averaged from 10replicatess.

PCR and sequencing was performed using GoTaq PCR Kits (Promega, Madison, WI, USA) in 50 μl volumes following the manufacturer's instructions, with reactions subjected to 2 minutes at 94°C, 35 cycles of 1 minute at 94°C, 1 minute at the appropriate annealing temperature (Table  2), and 1 minute at 72°C, followed by 10 minutes at 72°C.

An example of this is how installation and service tasks can be reported immediately, allowing the finance department to create the appropriate invoice within minutes.

She suspects that the firm delayed and postponed hearings in the hope that the family would back down, before settling, with the local authority agreeing to fund the place at the appropriate special needs school minutes before the tribunal was due to begin.

Cells were then incubated on ice with the appropriate antibodies for 30 minutes, washed, and sorted using an LSR II instrument and analyzed using FlowJo software.

For assays involving inhibitors, IFNγ primed macrophages were incubated with the appropriate inhibitors for 30 minutes before addition of target cells.

The cell preparations were incubated with the appropriate antibodies for 60 minutes at room temperature.

After blocking, sections were incubated with specific primary antibody at the appropriate concentration for 60 minutes at room temperature.