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The test bottle was fully mixed and put into the Microtox Toxicity Analyzer (DXY-2).
A sample of ZnO NW arrays on paper substrate (1 cm2) was immersed in a test bottle containing 114 μM MB (16 mL).
A 13-W UV lamp operating at a wavelength of 365 nm was used as a radiation light source; it was placed 15 cm from the test bottle.
Then, the solution was cooled to room temperature in air, and the test bottle was inversed to see if a gel was formed.
After that, the solution was heated in a water bath at a temperature of 80°C for 15 min. Then, the solution was cooled to room temperature in air, and the test bottle was inversed to see if a gel was formed.
And, there's the possibility that one day we'll be going toilet-to-tap for drinking water, an idea floated in the film by a deadpan, lab-coated Jack Black, holding a beta test bottle of "Porcelain Springs".
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As the test bottles did not show a reddish coloring, they assumed anaerobic conditions.
Test bottles will not be processed in the absence of a signed informed consent.
The final drug concentration in the test bottles was 1 μg/ml for INH and RMP, and 2 μg/ml for EMB.
To minimize the spillage of liquids during the sucrose test, bottles are filled in advance and kept in an up-side-down position for at least 12 hours prior to testing.
Now anti-doping authorities demand that competitors urinate into two testing bottles in front of a control officer, who then applies tamper-proof seals to the containers, which are individually labelled and sent by courier to the laboratory.
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