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To investigate optimal test vial (TV) volume, utility and reliability of TVs, intermediate temperature exposure (−88°C to −93°C) before cryostorage, cryostorage in nitrogen vapor (VN2) and liquid nitrogen (LN2), and long-term stability of VN2 cryostorage of human semen.
The biopsy was placed in a test vial, incubated at 37°C and read within 24 h.
Three to ten milligrams of substance were scraped into a test vial and weighed with an analytical balance.
Single females were allowed to acclimate to their test vial for 24 hours before a single male was introduced by aspiration.
Nematode-suspension (0.5 ml, 20,000 individuals) was transferred to each test vial and the test organisms exposed for 48 h to the respective treatment.
For EXTEM, 20 μL of 0.2 mol/L CaCl2 (star-tem™) and 20 μL of human recombinant tissue factor (r EXTEM™) were added to a test vial.
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Maybe it was a hoax (the threatening letter arrived on April Fools' Day); could be the test vials were somehow contaminated.
No oxygen depletion occurred in the test vials and Henry equilibrium was found to be established after 6 h.
Mr. Stewart, though, reacted the way many of us did upon encountering the mysterious visuals that accompanied the genome story on every broadcast (including "The Daily Show") -- indecipherable computer images, acres of test vials, weird helixes and symbols.
The method evaluation process considered various methodological issues such as partitioning of the toxicant between the water and the gas phase (Henry equilibrium), the possible depletion of oxygen in closed test vials, as well as microbial biodegradation of the test substance.
The chamber would contain a few test vials containing simple DNA messages for future cryptographers to decipher.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com