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Analysis of the experimentally determined miRNA targetomes indicated utilization of non-canonical target pairing.
Taken together, this data strongly suggests miR-503 may utilize supplementary target pairing for repression.
There were no differences between compensatory target pairing sites and the intact 5′ seed alone for both miR-503 and miR-103, making it difficult to draw conclusions as to utilization of compensatory target pairing.
However, target prediction is very challenging, especially when a high level of mismatches exists in miRNA:target pairing [ 49].
We went on to experimentally confirm that 3′ target pairing was necessary for miR-503 to repress DDHD2.
Additional file 3: Using RIP-ChIP and microarray gene expression profiling to explore miRNA target pairing outside of the canonical miRNA 5′ seed match.
Similar(42)
We analyzed predicted DTIs to identify target pairs whose predicted drugs may have synergistic bactericidal effect.
And for every TOMS for Target pair of shoes sold, a pair will go to a child in need".
In particular, for every minimotif in our database we generated all the (source, target) pairs.
We wished to validate additional miRNA:mRNA inverse target pairs.
Figure 2 displays these miRNA and target pairs.
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Justyna Jupowicz-Kozak
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