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These synthetic selection diagrams are obtained for the three tested materials.
To apply the directed evolutionary approach to engineer the 3-HP production system, we constructed a synthetic selection device using a 3-HP-responsive transcription factor and developed a selection approach for screening 3-HP-producing microorganisms.
Isolated yeast transformants were grown on synthetic selection media containing 2% dextrose without salt (see additional file 5: composition of synthetic selection media) for 36-72 h.
Cultures were grown in YPD, or if cells contained plasmids, in synthetic selection medium.
Twenty thousand yeast transformants were picked and inoculated into sterile 96 well U-bottom microtitre plates (Nunc, USA) containing synthetic selection media (see additional file 5: composition of synthetic selection media).
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The co-transformants were selected on synthetic complete selection medium lacking Leu, Trp and His.
This paper presents the extensive upgrades of Aspen Batch Process Developer™ (ABPD) that is a recipe-based batch process modeling and simulation environment, designed to employ over the entire life cycle of processes from early synthetic route selection to plant scale engineering.
Now, the questions to be asked are whether we can readily use such data to build expressive unit selection synthetic voices [5] and will the synthesis be good?
For selection, synthetic complete (SC) media lacking Leu and Trp (SC-Trp-Leu) or lacking Leu, Trp, His and adenine (quadruple dropout, QDO medium) were used.
Similarly to feature selection, synthetic data generation has received a considerable attention in the recent generic machine learning and data mining literature, especially in the context of data streams and concept drift (please see Bifet et al. 2011 and references therein).
In transformation experiments, protoplasts of the transformant candidates were regenerated in synthetic-defined (SD) selection medium (1% polypeptone S (Wako Pure chemical, Osaka, Japan; w/ v), 2% glucose (w/ v), 4.68% NaCl (w/ v), 2% agar (w/ v), 0.5 mM arginine, and 2 μg/ml Aureobasidin A (TaKaRa Bio, Shiga, Japan)) at 37°C.
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