Other test substrates promoted a pathological, synthetic phenotype of HCASMC and/or hyperproliferation of HCAEC.
The synthetic phenotype between sod1 and pri2-ts points to a connection between oxidative stress and DNA replication initiation.
In addition, Ca2 + signals regulate VSM gene transcription, proliferation, and migration of dedifferentiated or "synthetic" phenotype VSM cells.
Synthetic phenotype VSM growth and hyperplasia are hallmarks of pervasive vascular diseases including hypertension, atherosclerosis, postangioplasty/in-stent restenosis, and vein graft failure.
The synthetic phenotype is necessary for cell proliferation on the tissue scaffold but the cells must ultimately assume a quiescent, contractile phenotype for normal vascular function.
RASMC responded to 10% equibiaxial strain by becoming less elongated, and immunohistochemistry suggested that stretched RASMC shifted to a more synthetic phenotype in comparison to static controls.
A strong positive correlation existed between these protein markers of the synthetic phenotype and phosphorylated ERK levels, and this relationship persisted across matrix geometries and compositions.
As expected, the combination of these mutations conferred a synthetic phenotype.
If the synthetic phenotype observed between sodCI and recA in the animal was due to the inability of the recA strain to induce SOS, then sodCI should also show a synthetic phenotype in the lexA non-inducible strains.
The synthetic phenotype at still higher doses might reflect additional defects due to impaired function by other CDK targets.
Taken together, the data above suggest that the synthetic phenotype observed in the recA sodCI double mutant is not a result of the defect in recombinational repair.
