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One prerequisite for both methods is sufficient amount of samples.
To obtain sufficient amount of samples for qRT-PCR analysis, total RNA isolated from bulge keratinocytes (∼200 ng from 105 cells) was amplified by the RiboMultiplier sense-RNA Amplification Kit (Epicentre Biotech).. Equal RNA amounts (∼2 µg) were used to synthesize cDNA with the Transcriptor Reverse Transcriptase (Roche) and oligo-dT primers following the manufacturer's instructions.
Of the 371 women eligible for the current study, 328 women provided sufficient amount of samples for DNA extraction.
However, studies in human have been less extensive than in mouse due to the difficulty in collecting sufficient amount of samples to comprehensively profile T cell differentiation over time.
We therefore admit that mtDNA analyses with highly degraded ancient samples are sometimes too risky because sequencing errors commonly occurred during analysis, and they could not be corrected easily by a due course of repeated experiments with a sufficient amount of samples.
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However, this standard addition technique for quantifying plasma samples by LC-MS/MS requiresufficientnt amount of sample to set the standard addition calibration curve and more work (two calibration curves) is needed.
This high flow rate was chosen to be sure to get a sufficient amount of sample for the analysis and to compare with human data where the same flow rate was used.
Direct endoscopic observation makes possible the direct collection of sufficient amounts of samples from the infected region for microbiological examination.
The majority of these methods use PCR to obtain sufficient amounts of sample to analyze.
Selection of the tomont stage, which is external to the host and sediments at the bottom of the aquarium, facilitated the collection of sufficient amounts of sample for this study.
Depending on the number of biological samples and their replicates sufficient amount of control samples are required for any microarray study.
Related(11)
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