Of these 13 pooled samples, 10 had adequate amounts of sample remaining for additional analysis and were chosen for stereoisomer-specific analysis to validate and compare to our previous findings of total HBCD levels.
Another limitation is the retrieval of adequate amount of the sample and hence the need for techniques with high specificity and sensitivity to measure small amounts of proteins [ 48].
Ten microliters of normal human sera was pre-mixed inside microcentrifuge tubes, immediately before the assays, to adequate amounts of the samples containing the inhibitors (salivary gland extracts, intestinal contents or haemolymph).
One microliter of normal human sera was pre-mixed inside microcentrifuge tubes, immediately before the assays, to adequate amounts of the samples containing the inhibitors (salivary gland extracts, intestinal contents, haemolymph or intestinal microvillosities/perimicrovillar membranes preparation).
Adequate amounts of the samples were kept to analyze for glucose, total protein, LDH, cholesterol (CHOL) and triglycerides (TGs) in pleural effusions and sera.
Addition of adequate amount of sodium carbonate to the sample solutions, in order to adjust the pH and ionic strength of solutions, directly effect of the extraction efficiency.
After analysing pERK and p53, samples with an adequate amount of tumor tissue (for a usable pAKT IHC scoring) were available from 35 patients only.
Samples obtained from an independent aSLE cardiovascular risk cohort with at least 12 months of complete prospective follow up were analyzed (if the samples contained an adequate amount of plasma), and clinical data and scored outcome measures were also studied [ 41].
The powder sample was soaked in adequate amount of methanol for three days at room temperature with infrequent stirring and shaking.
