Site assignment of methionine oxidation using this workflow has two major drawbacks: (1) a substantial amount of sample is required to allow variant fractionation and subsequent peptide mapping (especially for low abundant variants) and (2) the procedure is time consuming, due to elongated sample preparation and LC-MS analysis.
However, this method is also limited because it cannot be used for the simultaneous monitoring of hundreds of miRNAs and requires substantial amounts of sample.
Neither PIEF nor OGE may be optimal because substantial amounts of sample peptides were left behind in the IPG gel matrix, as assessed by imaging of Cy3-labeled peptides remaining in the IPG strips after either analysis (results not shown).
However, a substantial amount of blood samples collected from a large number of individual animals is a prerequisite to ensure the diversity of the naïve library.
Affinity-based techniques require a substantial amount of starting sample, and their efficiency depends on the density of methylation marks within each specific fragment [ 43].
However, a substantial amount of this sample was not part of the Wave 1 parent interviews or measured for waist circumference and height at Wave 4. In addition, genome-wide association data is available on the sibling pair (SP) sample only.
In 1601, Hans Heinrich Nüschler signed a contract with his patron, Duke Friedrich of Württemberg in Stuttgart, to demonstrate his process for extracting a substantial amount of gold from a sample of silver.
Interaction of the accelerated ion beams (from the focus region) with the substrate, releases the substantial amount of energy which rises the samples temperature.
A substantial amount of health survey research makes use of samples drawn from landline telephone numbers using random digit dialling (RDD) [ 5, 7].
In particular, the two factors that we found significant determinants of the precise localization of CO events the avoidance of exons and the enrichment of MM2 do not appear to apply to GC events (with the caveat that there is not a substantial amount of statistical power in our sample).
