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Strain performance was also evaluated in the presence of 0.5 mM GalNAc and 2 mM phosphatidylcholine.
Rational metabolic and cellular engineering approaches are useful in improving strain performance.
The strain performance and downstream process greatly impact the economics of biobutanol production.
Evolutionary methods to improve strain performance are a powerful complement to rational, predictive strain engineering.
In summary, the deletion of APJ1 in GSE16 was not detrimental for the strain performance when fermenting spruce hydrolysate.
Although richer nutrients and more initial cells improved the fermentation of the hydrolysate, strain performance was still a distinct disadvantage.
Similar(17)
It is therefore clear that competitiveness of microalgal biodiesel will depend on improvements in both cultivation and harvesting technologies [ 4] as well as in strain performances [ 3].
Improving microalgal strain performances requires a better understanding in model microalgae of the mechanisms and regulations of carbon fixation, carbon allocation between biosynthetic pathways and induction by stresses.
However, in some cases such drastic modifications result in deteriorated strain performances, as the resulting flux distribution of such an intervention might not be optimal anymore, due to the interplay of the metabolic pathways in the producer strain.
Our data show that a combination of these conventional approaches together with genome and transcriptome analysis can help to sort out mutations, improving strain performances, and provide further targets for genetic engineering.
In this work, the strain E. coli LA02 was engineered for isobutanol production by combining NADP+ dependent Ehrlich pathway and biosynthetic KIV precursor pathway, but, as the key limitation for isobutanol production, the imbalance redox status with excess NADH and less NADPH supply should be solved to improve the strain performances.
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