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Before adding cell suspension onto the slide, cover slip is gently removed (Simon et al. 2013).
After that, a small drop of 1% acetocarmine was added and covered with a slide cover, and observed under the microscope (Motic BA200) after 3 5 min.
Rice anthers removed from the floret and squashed with the forceps onto the glass slide, and then added a small drop of 10 mg/L−1 eosin B (C20H6N2O9Br2Na2, FW 624.1, a tissue stain for cell granules and nucleoli) solution (dissolved in 4% sucrose), covered with a slide cover and observed after 10 min.
100 μL of spore suspensions of both B. subtilis ATCC 6051 and B. pumilus SAFR-032 were each deposited onto three sterile microscope slide cover slips; these cover slips were allowed to dry at room temperature for 24 h in a laminar air flow chamber.
Diatom cells were grown on the surfaces of microscope slide cover glasses, microscope slides or in small four-place bioreactor/culture slides (Becton-Dickenson, Franklin Lakes, NJ, USA) for 2 d and then treated with 10 mM EGTA, pH 7.8 or 0.2 M EDTA, pH 7.25 for 25 min.[32] The cells were washed with medium and observed with dark phase optics or after staining.
It uses an excellent slide cover that you control with a push-button, which has a solid snap to it and works extremely well.
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Rock slide covers monkey (fake monkey).
Each section was placed on a clean slide, covered with a cover slip, and then mounted with Canada balsam.
Probe/Hyb mix (200 μl) was used per slide, covered with glass coverslips.
The cell suspension was placed onto a microscopic slide covered by a coverslip.
Afterwards, they were mounted in glycerol on a slide, covered with a cover slip and sealed with nail polish.
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