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Exact(5)
However, the median size of insert clone is around 40 kb in paired-end sequencing by Kidd et al., which would make detection of small CNVs more challenging.
Though such vectors have the advantage that they can spread systemically within a plant and can be readily transmitted in order to bulk up material, they suffer from disadvantages in terms of the size of insert which can be stably incorporated and raise issues of biocontainment.
The libraries were tested for the presence and the size of insert by PCR using two primer pairs.
We picked 4 isolated colonies for each reaction and verified them for the chloromphenicol antibiotic sensitivity and the size of insert by colony PCR.
To analyze the size of insert DNA fragments in this library, 174 randomly selected clones were grown in 10 ml LB containing 12.5 μg/ml chloramphenicol, and the BAC DNA was isolated by alkaline lysis [ 33].
Similar(55)
The number of proviral insertions and size of inserts in individual G1 birds was analysed by Southern blot transfer.
However, the maximal size of inserted sequences can be estimated by summing the lengths of the unaligned reads and dividing by the average coverage.
The average size of inserts was 500 bp.
This is used to check the size of inserts within BAC samples.
The clones were retrieved, the DNA extracted, and NotI digests were run on PFGE to estimate the size of inserts.
Among 8 YAC clones characterized by CHEF electrophoresis, size of inserts varied from 50 to 200 kb.
Related(20)
length of insert
magnitude of insert
size of insertion
range of insert
depth of insert
importance of insert
size of combined
size of suggested
size of registered
size of injecting
size of participating
size of existing
measurements of insert
size of intervening
size of inserts
size of block
size of bird
size of shop
size of dog
size of trailer
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