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The number of proviral insertions and size of inserts in individual G1 birds was analysed by Southern blot transfer.
The average size of inserts was 500 bp.
This is used to check the size of inserts within BAC samples.
The clones were retrieved, the DNA extracted, and NotI digests were run on PFGE to estimate the size of inserts.
Among 8 YAC clones characterized by CHEF electrophoresis, size of inserts varied from 50 to 200 kb.
The PCR test results showed that the size of inserts was between 1 3 kilobases, the library reorganization was 97.85% and the no-load rate was 2.15%.
Similar(51)
However, the maximal size of inserted sequences can be estimated by summing the lengths of the unaligned reads and dividing by the average coverage.
However, the median size of insert clone is around 40 kb in paired-end sequencing by Kidd et al., which would make detection of small CNVs more challenging.
The libraries were tested for the presence and the size of insert by PCR using two primer pairs.
Secondly, the duplicated regions contain the varied numbers of repeat motifs which result in distinct size of inserted fragments among 1Sx subunits, 1Dx2.2 and 1Dx2.2*.
We picked 4 isolated colonies for each reaction and verified them for the chloromphenicol antibiotic sensitivity and the size of insert by colony PCR.
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