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For a fixed iodine concentration of 5 mg/ml and reconstructions maximizing overall accuracy across the range of insert diameters, the minimum diameter classified with accuracy >80% was ∼ 15 mm for FBP and PLQ and ∼ 10 mm for PLTV, improving to ∼ 7 mm for FBP and PLQ and ∼ 3 mm for PLTV at 15.6 mGy.
Prior to the arrival of NGS platforms, WGS on larger genomes was possible because Sanger-based sequencing platforms generated paired-end reads of 700 bp or greater and a range of insert sizes from 3 to 40 kb (Weber and Myers 1997).
Its major feature was broad range of insert size and very low fraction of PE reads.
In consequence, it remains unclear, if the sub-libraries actually contained the expected range of insert sizes.
A Colony-PCR was performed to assess the range of insert sizes as well as the cloning efficiency.
A big insert of 465 nt in the middle of the cluster created no putative tRNA structure in the range of insert.
Similar(48)
With this range of inserts an appropriate library size for the screen was estimated after Clarke and Carbon.
And, in addition to that, the host expression machinery might be tuned to a broad range of inserts, with varying fragment types, origins and sizes.
Due to a newly designed type of polylinker, directional cloning of a wide range of inserts is greatly facilitated, and HA-tagging of the insert of interest is possible if needed, depending upon which pPRIG vector is selected.
Comparison of the size range of inserts observed in traditional and "gel-free" Fosill libraries indicates highly similar performance (mean insert size = 38.0 and 36.7 kb, respectively; Figure 2).
wgsim from Samtools[ 23] was modified to simulate data with a wide-range of insert sizes and various levels of heterozygosity (http://sourceforge.net/projects/anytag/files/).net/projects/anytag/files/
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