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Purified and sequenced construct was transformed into BL21 DE3 chemically competent cells and expressed using approaches from previous standard procedures with minor alterations, such as the addition of a 10 min, 42 °C heat shock step prior to induction.
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The 81 sequenced constructs (all different) and 87 constructs analyzed by restriction digest but not sequenced were transformed in Agrobacterium strain GV3101 pMP90.
Protein sequences were translated from BAC clones or sequenced constructs using Expasy translate, and aligned by hand.
The sequenced constructs were used for transient transfection of A. thaliana protoplasts as described previously (Cardinale et al, 2002).
A pseudomolecule is a sequencing construct that ideally corresponds to the chromosomal DNA molecule.
Primers were designed to amplify the full sequencing construct in a single PCR reaction.
The non-normalized frameshift efficiencies for the native sequence construct in all biological replicates (n = 9) was 5.4 ± 0.9%.
A contiguous region of DNA sequence constructed by aligning many sequence reads.
Hong builds moments of extraordinary romantic power, culminating in a brilliant sequence, constructed from a single ten-minute shot parsed with brisk and assertive zooms and pans, in which Young-hee reflects on her bitter experiences.
Fig. 5 Scenario A. Stream sequences constructed by ASNSSS.
We tried to revise the original spliced sequences constructed at seven sites drilled during Exp.
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