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Sample concentration providing 50% inhibition (IC50) was calculated by plotting the inhibition percentage against sample concentration.
Each sample concentration and a control was replicated three times.
Sample concentration providing 50% inhibition (IC50) was calculated from the graph plotting inhibition percentage against sample concentration.
The sample concentration providing 50% inhibition (IC50) under the assay condition was calculated from the graph of inhibition percentage against sample concentration.
There is a clear distinction between the signal intensity and the logarithm of the sample concentration.
The focal length is critical for the design of micro sample concentration devices.
The system was linear (R2>0.99) within the working sample concentration and sample volume ranges.
Each sample concentration was previously estimated using Qubit methodology (Invitrogen, USA).
After sample concentration the dispensing granule came into being from water extracts by freezing drying.
Dilutions were carried out so that sample concentration fall within the calibration curve.
The DNA sample concentration was determined by a Nanodrop (Thermo Scientific, USA).
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