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The testing concentration of the compounds was set based on the prior cytotoxicity of other cancer cell lines.
As mentioned in "Testing concentration and competition", the ALI does not allow for comparison in absolute terms, but only trend comparison among countries.
At the low testing concentration (1∶1000), the bees responded to limonene as well as to the training mixture (McNemar test [2×2 Table] with Bonferroni corrected threshold; Chi square = 0.25; df = 1; p = 0.617), but significantly less to myrcene (p = 0.004) and β-pinene (p = 0.003).
A testing concentration of 100 μg/mL and exposure time of 24 hours was used.
This allowed us to obtain the required testing concentration for each of the HPV assays.
Intellectual Quotient (IQ) derived from the standardised MENSA question bank assessing spatial awareness, memory testing, concentration, and subgroup classification [ 19 ].
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At higher testing concentrations bees responded to all odorants equally.
In some cases, this meant testing concentrations in the cytotoxic range.
Stage 2: Test for activity at lowest tested concentration.
Each test concentration was performed in triplicate.
Therefore, a limit test with several test concentrations instead of only one test concentration is preferred to obtain information about the dose response relationship.
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