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Smear microscopy was performed using a conventional fluorescence microscopy after specimen concentration using high-speed centrifugation.
Pre-specified secondary analyses included an assessment of correct treatment among those with smear-negative, culture-positive TB at 2 weeks, impact of specimen concentration on sensitivity and specificity among adults and children, and differences between HIV-infected and uninfected participants.
Volume of 100 μL of the specimen (concentration 1 mg/mL) was added into the agar well in the center of the Petri dishes and left to incubate for 2 hours at room temperature; afterwards the plates were transferred to the 37°C for 18 h.
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The assay is rapid for specimen concentrations ≥1 mg/l and is easily tuned to achieve low quantification limits at high chromatographic resolution for lower concentrated samples.
The nature of the sample introduction technique is dependent on numerous factors, including the type and form of the sample specimen, the concentration levels, scope and chemical form of the analytes to be determined, and the quantity of sample available for analysis.
Specimens for dynamic melt rheology were produced by ultrasonicating the nanoparticles (at a specimen-specific concentration relative to the copolymer) for 30 min in toluene to achieve a satisfactory dispersion, followed by copolymer dissolution and further ultrasonication, and then air- and vacuum-drying, all performed at ambient temperature.
In CCP specimens, stress concentration and free-edge effects produced a small level of notch weakening.
To improve sensitivity, multiple specimens and concentration procedures, as well as a variety of staining methods, are needed to achieve ample sensitivity [ 13, 14].
In contrast to 5-fluoro-ADB-PINACA in the specimens, the concentrations of MAB-CHMINACA were found at much higher concentrations than those of 5-fluoro-ADB-PINACA.
In these specimens, protein concentrations of 0.28±0.12 g/L yielded reproducible MALDI-TOF MS profiles that differed by 1 17 USSPs (Table S7).
For plasma specimens, undetectable concentrations were treated as half the lower limit of detection to allow inclusion in intergroup comparisons.
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