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High magnification of VEGF-delivered, HSC and MSC co-transplantation sample showed robust collagen deposition and areas of apparent bone trabecula-like structures (Fig. 6D2).
Fibroblast activation results in synthesis of a high levels of ECM, and during the first days of the healing process, a new robust collagen "scaffold" is produced that directs and regulates cell migration and guides new vessel formation.
Moreover we observed a less robust collagen lattice structure in dermal equivalents with KSS fibroblasts in comparison to NHF by picrosirius red staining of histological sections.
Despite robust collagen gene induction in the livers of MCD-fed mice, there was no increase in smooth muscle-alpha-actin expression (Fig. 3c).
Figure 3 highlights the decreased ordering in MMP13 KO mice, with WT mice demonstrating many robust Collagen I fibers more frequently oriented parallel to the tumor boundary, closely resembling a TACS-2 arrangement as previously described [ 5, 12, 19].
Robust collagen deposition along with strong alpha-smooth muscle actin (α-SMA) expression was observed in a significant number (5 out of 12) of FoxM1b Tg Arf−/− mice (Fig 1B).
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Our identification of the collagen receptor GPVI as another binding site for SSL5 provides, along with GPIbα, a mechanistic basis for understanding SSL5 induced platelet activation, as GPVI is known to couple with the robust collagen-induced signalling pathway [21].
However, these mice exhibit more robust accumulation of collagen in response to silica.
While conditioned medium from SCs had minimal effect, CMC-CM dramatically reduced TGF-β-induced fibroblast 3H-proline incorporation, a robust marker of collagen production (Fig. 1).
In unchallenged endothelial cells, fibrillar collagen induced robust capillary morphogenesis.
The aggregating proteoglycan along with type II collagen provides robust mechanical properties to cartilage in a healthy joint.
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