IL-1β stimulated 786-0 cells were embedded within a semi-solid collagen matrix impregnated with a FITC-collagen conjugate.
After incubation for 48 hrs at 37°C, solid phase collagen and cells were pelleted and the supernatant analyzed for FITC using a Perkin-Elmer Victor V multilabel counter [ 31].
Equally solid production of collagen among the entire cell population served as an evidence of the cells' uncompromised ability to form extracellular matrix following growth on PCL/HAp films.
The triphasic mechano-electrochemical theory [ 25] applied here considers a charged hydrated soft tissue as a mixture that consists of a porous permeable charged solid phase (ECM, collagen fibers, and PGs), a fluid phase (water), and an ion phase with two monovalent species (Na+ and Cl−).
As seen in the QCM-D traces (Fig. 1) after H*protein adsorption, at 13,000 s (point D), solutions of collagen (solid line), BSA (dashed line), and casein (dotted line) were injected respectively and allowed to adsorb for 2 h before rinsing again with buffer (point E).
CMs that were relatively thick when dry exhibited a greater proportional increase in rehydrated thickness and a greater diffusivity (when rehydrated) to 4.3 kDa dextran than did CMs that were relatively thin when dry. Hollow, tubular CMs of several configurations were prepared by embedment of solid, removable forms into collagen hydrogels prior to drying.
But, at least in America, we don't care when our packaged foods contain things like gelatin, which Google describes as "a translucent, colorless, brittle, flavorless solid substance, derived from collagen obtained from various animal by-products".
Soft tissue cells contain about 10%to15%5% protein solids, and soft tissue collagen is less densely aggregated than in bone and dentin matrix.
MM carried out the solid-phase MMP-collagen binding studies.
