The assay for each matrix was linear within the quantification range with determination coefficient (r2) values above 0.995.
It is widely used because of its high sensitivity, good reproducibility and wide dynamic quantification range (Pfaffl, 2004).
The serum assay showed good linearity within the quantification range 0.25 - 100 ng/mL for both stanozolol and 3′-hydroxystanozolol, with determination coefficient (r2) values higher than 0.9981.
Regression analysis indicated that the assay showed excellent linearity within the quantification range of 0.5 to 200 pg/mL water for stanozolol.
Correlation coefficients were greater than 0.99 for all nucleosides, such that the calibration curves showed good linearity within the quantification range.
The assay showed a wide quantification range of 5 log units using calibrated cell suspensions of Pediococcus parvulus 2.6 and Oenococcus oeni I4.
A reflected fiber optic sensor for explosives analysis was developed, with low sample consumption (20 100 nL) and a wide concentration quantification range (1.1 250 mg L−1).
Based on the data summarised in Table 5, the proposed electrochemical DNA biosensor based on PSA-AuNPs immobilisation material shows an exceptional broad linear quantification range compared to other planar two-dimensional electrodes as the DNA supporters.
The determination the activity of alkaline phosphatase in serum samples enabled a quantification range of 0.3 mU/L–1 U/L with a detection limit of 0.1 mU/L.
The quantification range of this system was 4 orders of magnitude, from 103 to 106 copies per PCR, corresponding to the detection limit of 300 target copies per mL.
