Quantification cycle values in different tissue (brain, heart, liver, muscle, spleen and head kidney) samples of Clarias batrachus.
ΔCq was designated as the mean quantification cycle of an mRNA in a tissue subtracted with the mean quantification cycle of a reference RNA in the same tissue.
Quantification cycle (Cq) data were obtained from each reaction with eight primer pairs.
Quantification cycle (Cq) values, for each sample, were determined to estimate the blood viral RNA load [20].
The standard curves were used to transform the quantification cycle (Cq) values to the relative number of cDNA molecules.
In determining the level of RT-qPCR amplified products, the quantification cycle was assumed to be proportional to the starting Armored HIV RNA molecules in each dilution assayed.
The variation of quantification cycle (Cq) across samples was compared among three reference genes (B2M, Actb, Rpl10a) to select the most appropriate reference gene for our study.
Data were collected as quantification cycle (Cq, formerly cycle threshold (Ct)) values using the iCycler iQ Optical System Software Version 3.1.
Cq, Quantification cycle.
Cq quantification cycle values for RT-qPCR.
