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Background was subtracted from the quantification area.
For quantification, area covered by the fluorescent intact cells was calculated after background correction for each image using ImageJ.
Computerized quantification of vessel density was then performed and expressed as a ratio from ischemic to non ischemic leg of the percentage of pixels per image occupied by vessels in the quantification area.
The quantification area is calculated numerically using the trapezoidal rule over 100 points.
Grids were placed on each image and the quantification area for each feature was adjusted for optimal placement.
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For quantification, areas of the images that did not include sensor nanofibers were excluded from the analysis.
Quantification included area, perimeter (a distance surrounding an area), circularity (a degree of roundness where the number 1.0 depicts a perfect circle), and Feret's diameter (the longest distance within an area) for each analyzed region.
Quantification of area without Plp1 transcript signal (demyelinated regions and areas associated with lesions) was performed as previously described [11].
For quantification, the area of the Cas3- or mRFP-positive cells was determined and normalized to the area of the ptc stripe.
For quantification, peak area ratios of the analytes to the internal standard were calculated as a function of the concentration of the substances.
For quantification, peak area ratios of the analytes to the internal standard d4-DEHP were calculated as a function of the concentration of substances.
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