We also performed computational modeling of these protein variants.
They have developed an evolutionary platform which can test 10 billion protein variants in one petri dish to evolve new therapeutics.
The startup claims its platform can test more than 10 billion protein variants in one petri dish to create new therapeutics.
Protein engineering is a common strategy for the generation of protein variants with new properties.
We also verified that these selected protein variants do not substantially bind to coated BSA protein (data not shown).
The protein variants were purified and tested for the solubility in the absence of detergent.
Phage display system allows selection of protein variants with improved binding characteristics (Azzazy and Highsmith 2002; Mullen et al. 2006).
To identify possible Atp7a protein variants, three distinct polyclonal antisera were utilized.
All the protein variants studied here refold with apparent two-state kinetics.
Combinatorial engineering involves expression of diverse libraries of protein variants coupled with high-throughput screening for desired phenotypes.
High-throughput screening of large libraries of protein variants is therefore becoming increasingly important in R&D.
