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Many internet retailers create quality variants of a homogeneous good by bundling it with several shipping options that differ by delivery time.
As a result of the limited sample size and strict criteria, we excluded low quality variants.
SNPs and indels with quality scores > 20 and depths > 15 were considered high quality variants.
After filtering the chr 3 region for exonic, rare, good quality variants we only see 4 mutations (Table 2).
The variant call format (vcf) output files generated by SAMtools were further filtered for low quality variants.
Thus, in order to create a list of high quality variants only homozygous calls were considered for further analysis.
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Our analysis also focused on the use of higher quality variant calls as was done in the 1000 genomes pilot study to evaluate the likelihood of a candidate variant call being a real event [37].
3,039 high quality variant sites were identified and used to create a maximum likelihood phylogenetic tree.
This protocol uses population and pedigree priors to improve estimates of genotype likelihoods, providing higher quality variant calls.
The Genome Analysis Toolkit (GATK) was used for recalibration of base quality, variant calling, filtration and evaluation [ 14, 15].
After obtaining a high quality variant set, we performed a genotype-refinement protocol, as described in (http://gatkforums.broadinstitute.org/discussion/4723/genotype-refinement-workflow .org/discussion/4723/genotype-refinement-workflow .org/discussion/4723/genotype-refinement-workflow
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